Phylogenetic Groups/B2 Subgroup Distributions, Serogrouping and Identification of Virulence Factors in Extended-Spectrum Cephalosporin-Resistant Escherichia coli Strains Isolated from the Stool of Healthy Children Under 10 Years Old

Background: Segregation of Escherichia coli (E. coli) into the phylogenetic groups was observed in experiments so that group B2 contained enteropathogenic E. (EPEC) strains and extraintestinal pathogenic (ExPEC). Objectives: This study aimed to identify extended-spectrum Cephalosporins resistant isolated from stool healthy children under 10 years old. Methods: One hundred broad-spectrum were collected feces 10. Subsequently, we grouped via PCR based on genes yjaA, chuA, arpA, as well, TspE4.C2. Then, according Clermont et al.’s study, used two individual multiplex PCRs for identifying sub-groups (I-X subgroups). Serogroup typing with 12 O-antigen analyzed PCR, finally, virulence (cnf1, papG, ibeA, malX, usp, cdt, eae, bfp, afa-Dr) identified PCR. Results: The age range between 1 years. unknown phylogroups most common this study. subgroups I (2%), IX (1%), V (8%), IV, V, VII IX, (3%), III, VII, (%1), (6%), each subgroup carrying distinctive sets ExPEC markers. results also showed 29% had malX 23% papGII. It found 32% antigens O2 36% unknown. Conclusions: In 27% belonged phylogroup 6% B1 phylogroup. Moreover, serogroups O2, O16, O25 predominant papGII, papGIII, aggR, eae highest lowest supply among tested strains, respectively. isolates shown have further virulence-related comparison non isolates.